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«“√ “√ √“™∫— ≥±‘ µ¬ ∂“π ªï ∑’Ë Û ©∫— ∫∑’Ë Ò ¡.§.-¡’ .§. ÚıÙ¯ 73 Somsak Damronglerd, et al. FIG. 2. Reduction of sulfonated azo dyes by Paenibacillus sp. strain A5 in the presence or absence of AQDS. The reduction of Remazol Brilliant Orange 3R (A), Remazol Black B (B), and Remazol Brilliant Violet 5R (C). FIG. 3. Anaerobic reduction of sulfonated azo dyes in the presence of different concentrations of AQDS by whole cells of Paenibacillus strain A5. The cells of strain A5 were grown aerobically in a minimal medium with glucose (5 mM). When cell reached the lated-exponential growth phase, cells were harvested by centrifugation and resuspended anaerobically in Na-K phosphate buffer (pH 7.5; 50 mM). This cell suspension (protein content, 0.09 g l -1 ) was completely filled into cultured tubes (10 ml). The oxygen-free reaction mixtures contained sulfonated azo dyes (0.1 mM), glucose (5 mM), and different concentration of AQDS. The decolorization of the sulfonated azo dyes was measured spectrophotometrically at λ max of each azo dye.