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«“√ “√ √“™∫— ≥±‘ µ¬ ∂“π ªï ∑’Ë Û ©∫— ∫∑’Ë Ò ¡.§.-¡’ .§. ÚıÙ¯ 71 Somsak Damronglerd, et al. TABLE 2. Effect of the different redox mediators on anaerobic reduction of sulfonated azo dyes by Paenibacillus sp. strain A5 Azo dye Redox mediator a Specific reduction rate b (µmol min -1 g of protein -1 ) with: Whole cells Membrane Cell extract Remazol Brilliant Orange 3R None 1.5 5.1 1.9 AQDS 2.2 9.4 2.2 FAD 1.6 6.5 4.8 Remazol Black B None 1.2 1.9 1.7 AQDS 1.7 2.9 2.4 FAD 1.3 1.9 3.4 Remazol Brilliant Violet 5R None 0.6 5.9 3.3 AQDS 1.0 10.2 5.0 FAD 0.7 6.4 7.0 a The redox mediators were added in concentration of 50 M each. The E 0 ’ values of AQDS and FAD are -184 mV and -210 mV, respectively. b Specific reduction rate of sulfonated azo dyes with whole cells, cellular membrane or cell extract was determined as described in Materials and Methods. TABLE 3. Specific activities of quinone reductase and flavin reductase in membrane-bound and cell extract of Paenibacillus sp. strain A5. a The enzyme activities were determined spectrophotometrically as described in Materials and Methods. Specific activity ( mol min -1 g of protein -1 ) a Membrane Cell extract Enzyme Quinone reductase 81.8 8.8 Flavin reductase 6.8 72.1