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สารฮิ วมิ กช่วยเพิ่ มความสามารถการลดสี ... 224 The Journal of the Royal Institute of Thailand Vol. 37 No. 1 Jan.-Mar. 2012 Abstract Humic substance enhanced anaerobic reduction of sulfonated azo dyes by Paenibacillus sp. strain A5 Somsak Damronglerd Department of Chemical Technology, Faculty of Science, Chulalongkorn University, Phayathai Road, Pathumwan, Bangkok 10330, Thailand Nuttapun Supaka Program of Biotechnology, Faculty of Science, Chulalongkorn University, Phayathai Road, Pathumwan, Bangkok 10330, Thailand Kanchana Juntongjin Department of Microbiology, Faculty of Science, Chulalongkorn University, Phayathai Road, Pathumwan, Bangkok 10330, Thailand Supat Chareonpornwattana Department of Microbiology, Faculty of Science, Chulalongkorn University, Phayathai Road, Pathumwan, Bangkok 10330, Thailand Marie-Line Delia LaboratoriedeGénieChimique,INP-ENSIACET,5,ruePaulinTalabotSiteBassoCombo-BP 1301, Toulouse 31106, France Pierre Strehaiano LaboratoriedeGénieChimique,INP-ENSIACET,5,ruePaulinTalabotSiteBassoCombo-BP 1301, Toulouse 31106, France The humic quinone moiety model compound anthraquinone-2,6-disulfonate (AQDS) could function as redox mediator in the unspecific anaerobic reduction of different industrially relevant sulfonated azo dyes by Paenibacillus sp. strain A5. This compound was enzymatically reduced by the cells of strain A5 to corresponding hydroquinone, which subsequently reduced the azo dyes outside the cells in a purely chemical redox reaction, and ultimately causing decolorization. Cell fractioning experiments demonstrated that the AQDS-dependent azo reductase activity was located in the cell membranes of strain A5. For strain A5, the presence of both membrane-bound and cytoplasmic azo reductase activities was shown, and they were probably different enzyme system, and the former system has significant importance in the reduction of sulfonated azo compounds in vivo, when quinoid redox mediators may be present. Possible applications of AQDS stimulated anaerobic reduction of azo dyes for the treatment of dye-containing wastewaters are also discussed. Key words: sulfonated azo dyes, azo reductase, anthraquinone-2,6-disulfonate, Paenibacillus sp.