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Preparation and Assessment of Chitosan Encapsulated Menthol Microcapsules for Leave-on Conditioners 104 The Journal of the Royal Institute of Thailand Vol. 37 No. 4 Oct-Dec 2012 Hair treatment analysis Optical micrographs in Figure 5 reveal surface morphology of the hair. The hair treated with the leave-on conditioner was smooth compared with the virgin hair. The arrow pointing to the position where the medulla of virgin hair was clearly observed as shown in Figure 5 (a) was compared with the treated hair with the similar angles [Figure 5 (b)]. The scale pattern reveals the hair skin where the cuticle edge of virgin hair is shown in Figure 5 (c). The optical micrograph of the treated hair in Figure 5 (d) shows the substance which stayed near and covered the cuticle edge was probably the conditioner (indicated by the oval line). The conditioner molecules containing cationic surfactants and chitosan molecules provide positively electrical charges to the conditioner. Figure 4 Temperature ( º C) Time ( S ) Temperature ( º C)