59-05-032 Proceeding

54 Proceedings of the Princess Maha Chakri Sirindhorn Congress IV. Conclusion The 50 kDa-sizedCMCase enzyme produced fromBacillus sp. strainMSL2-S2was identified by SDS-PAGE and zymogram analysis. The enzyme has optimal temperature of 55°C and optimal pH of 6.0. Generally, industrial processes are carried out at high temperatures, therefore this CMCase with stability at high temperature offers the possibility to be applied in bioconversion in industrial application [9]. However, further investigation is needed, especially, on the enzyme kinetics, and production optimization to make it become feasible in economic consideration. Acknowledgement The authors would like to thankKingMongkut’s University of Technology, North Bangkok (Research University Grant No. KMUTNB-GOV-57-41, 58-22, and 58-27) for financial support of this work. References B. Solomon, “Biofuels and sustainability,” Ann N Y Acad Sci. 2010, vol. 1185, pp. 119-34, 2010. P. Kumar, Barrett DM, DelwicheMJ, and Stroeve P, “Methods for Pretreatment of Lignocellulosic Biomass for Ef cient Hydrolysis and Biofuel Production,” Ind. Eng. Chem. Res., vol. 48, pp. 3713-29, 2009. L. Lynd, Weimer PJ, van Zyl WH, and Pretorius IS, “Microbial cellulose utilization: Fundamentals and biotechnology,” Microbiol Molec Biol Rev, vol. 66, pp. 506-577, 2002. S. Sadhu andMaiti TK, “Cellulase Production by Bacteria: AReview,” BritishMicrobiology Research Journal, vol. 3, pp. 235-258, 2013. Y. Zhang, Himmel ME, and Mielenz JR, “Outlook for cellulase improvement: Screening and selection strategies,” Biotechnol Adv, vol. 24, pp. 452-81, 2006. M. Sriariyanun, Bangkha P, Chaisamaung A, Thanachan W, Chaijuckam P, Kangsadan T, and Klinchan S, “Characterization and purification of cellulase enzyme produced by Bacillus Figure 4 The CMCase activity of cellulase fraction No. 13 at different temperature.