59-05-032 Proceeding

49 Proceedings of the Princess Maha Chakri Sirindhorn Congress This paper demonstrates the feasibility of using cell rolling velocity in microchannel to characterize distinct phenotypes of neutrophil altered by various kinds of inflammatory stimulus. Presented approach can not only differentiate healthy neutrophil from treated neutrophil, but also separate different kinds of stimulus treated neutrophil. However, many improvements need to be fulfilled in order tomake this approach possible to use in clinical testing. Number of cells flowing through image capture region is uncontrollable possibly leading to insufficient data and incorrect data interpretation. Moreover, more precise velocitymeasurement also can be achieved by increasing sampling frequency (less than 0.5 sec). Another by product is that the treated neutrophils in other flow layers can be observed. References E. Galkina and K. Ley, “Leukocyte recruitment and vascular injury in diabetic nephropathy,” Journal of the American Society of Nephrology, vol. 17, pp. 368-377, 2006. A. Kantar, P. L. Giorgi, G. Curatola, and R. Fiorini, “Alterations in membrane fluidity of diabetic polymorphonuclear leukocytes,” Biochemical Medicine and Metabolic Biology, vol. 46, pp. 422-426, 12// 1991. M. M. Hartge, T. Unger, and U. Kintscher, “The endothelium and vascular inflammation in diabetes,” Diabetes and Vascular Disease Research, vol. 4, pp. 84-88, 2007. M. Delamaire, D. Maugendre, M. Moreno, M. C. Le Goff, H. Allannic, and B. Genetet, “Impaired leucocyte functions in diabetic patients,” Diabetic Medicine, vol. 14, pp. 29-34, 1997. H. W. Hou, M. E. Warkiani, B. L. Khoo, Z. R. Li, R. A. Soo, D. S.-W. Tan , et al. , “Isolation and retrieval of circulating tumor cells using centrifugal forces,” Scientific reports, vol. 3, 2013. Figure 8 Overlaid distribution curve of all neutrophils set