59-05-032 Proceeding

48 Proceedings of the Princess Maha Chakri Sirindhorn Congress Distribution curves of all neutrophil sets are overlaid and shown in Figure 8. Interestingly, overlaid curves show that the different sets of neutrophil has distinct differences in cell rolling phenotypes. Figure 6 Rolling velocity distribution of neutrophils treated by glucose Figure 7 Rolling velocity distribution of neutrophils treated by PMA Similar to TNF- α treatment, velocity distribution of glucose-treated neutrophils spread ranging from low speed (0.5 µm/s) to high speed (15 µm/s). From Figure 7, PMA-treated neutrophils remained firmly attached or moved at very low speed (less than 0.5 µm/s).