59-05-032 Proceeding

47 Proceedings of the Princess Maha Chakri Sirindhorn Congress Average rolling velocity of every cell were analyzed and their distributionwere represented using histogramplots.The histogramand distribution curve of collected rolling velocities of healthy neutrophils is shown in Figure 3 while neutrophils treated with TNF- α , glucose and PMA are shown in Figure 4, Figure 5 and Figure 6, respectively. All histograms were plotted with equal bin size width of 0.5 µm/s.The following table shows the number of cellsmeasured in our experiments. Table 1  Number of observable cells in each set Set Healthy TNF- α Glucose PMA number of observed cells 346 280 67 54 Healthy neutrophils had a tight velocity distribution with a range between 2 µm/s to 4.75 µm/s and their trajectories were well connected as shown in Figure 3 A. Figure 4 Rolling velocity distribution of healthy neutrophil Figure 5 Rolling velocity distribution of healthy neutrophils treated by TNF- α FromFigure 5, velocity distribution of TNF- α treated neutrophils wasmore heterogeneous with a low speed (less than 0.5 µm/s) to high speed (18 µm/s).