59-05-032 Proceeding

379 Proceedings of the Princess Maha Chakri Sirindhorn Congress Figure 3 Features and similarities in alignment window of The 19 sequences of Thailand Tamarind cultivars after conducted multiple alignment in ClustalW. These datum indicated that their single nucleotide sequence in partial rbcL gene were variated or point mutated and they can be use as DNA Barcode for cultivar identification of Thai Tamarinds. Nevertheless, they have been in concordance with research of Patipanee Khunthapok, 2007[6], which presented in dendrogram as shown in Figure 1. 4. Conclusion 1. Fromphylogenetic reconstruction study showed that the 19Thailand tamarind cultivars were divided into 19 groups concordance with the tamarind derived cultivars Phetchabun opinion in Phetchabun Province, Thailand. 2. T. indica Chloroplast rbcL gene for ribulose -1,5-bis phosphate carboxylaese /oxygenase large subunit, partial CDS 611-712 bp could be used as molecular maker for classification and identification of Tamarindus indica L. in both species and cultivar levels. By comparison of single nucleotide sequences using ClustalW and Blatn software and can be use as DNA Barcodes. For sustainably crop improvements and developments in Thailand. 5. Acknowledgement This project is supported by the annual Government statement of expenditure 2014, Assoc. Prof. Dr. Pruang Junda, the Chancellor of Phetchabun Rajabhat University and Dr. Ratana Chayaratanasin, the Director of Phitsanulok Artificial Insemination and Biotechnology Research Center for supporting N 2 liquid to this project.