59-05-032 Proceeding

376 Proceedings of the Princess Maha Chakri Sirindhorn Congress The 19 cultivars of Tamarindus indica. L. were Srichompoopling; (SCPP), Srichompooklom(SCPK), Seetongnug; (STN), Seetongbowm; (STB), Seetongjook; (STJ), Khuntee –yourk; (KTY), Khuntee –soy; (KTS), Sripakdee; (SPD), Sangartit; (SAT), Prakaiphet; (PKP), Preao–puenmuang; (PPM), Praroj; (PRR), Numpueng; (NUP), Intapalum; (ITPL), Prakaitong; (PKT), Preao–yak; (PRY), Fagdap; (FAD), Muenjong; (MUJ), and Phetbourtong; (PBT). 2.2 DNA extractions DNA extractions and normalizationwere performed by C-TABmethod[1], using Real Genomics Kit, RBC Bioscience Crop, Taiwan. 2.3 PCR amplification and detection PCR amplifications are performed using 2 primers which were designed by Macrogen Ltd., Korea.The two primers are RbcL-Tam-F03 and RbcL-Tam-R04.[2]. PCR products were detected through 1% gel –Electrophoresis and send to sequencing. 2.4 The DNA sequencing of 19 tamarinds were aligned by NJ method for phylogenetic tree reconstruction[3, 4]. As shown in figure 1. 2.5 Using ClustalW software in BioEdit. Then single nucleotide of each cultivar was analyzed using BLASTn from NCBI Databases compared to T.indica rbcL gene from GenBank. As shown in table 1. 3. Results and Discussions Table 1 Accession No. AB378732.1 the nucleotide sequences of rbcL gene of T. indica L. and the nucleotide sequences of different 19 cultivars (Thailand T. indica L.). Nucleotide Sequences of rbcL gene from 19 Tamarind cultivars (partial CDS) No. of bp Site to site of partial gene that compared to complete gene of T. indica L. from GenBank T. indica rbc L gene. (complete CDS) 1398 1………1398 1. Srichompooklom 641 688……1346 2. Srichompoopling 671 744……1359 3. Seetongbow 611 731……1347 4. Seetongnug 621 731……1351 5. Seetongjook 649 704……1344 6. Khuntee-yourk 643 706……1347 7. Khuntee-soy 649 703……1349 8. Sripakdee 678 734……1386 9. Sangartit 671 692……1337 10. Prakaiphet 646 734……1372 11. Preaopuenmuang 670 700……1344 12. Praroj 645 706……1347 13. Numpueng 712 732……1394