59-05-032 Proceeding

375 Proceedings of the Princess Maha Chakri Sirindhorn Congress DNA BARCODES FOR CULTIVAR IDENTIFICATION OF THAILAND Tamarindus indica. L. Benchaporn Srisuvoramas 1 , Surachest Aiumsamang 1 Sompong Sansenya 2 and Lertharong Sripanom 2 1 Biology Department, Faculty of Science and Technology. Phetchabun Rajabhat University 2 Department of Chemistry, Faculty of Science, Rajamangala University of Technology, Thanyaburi Abstract: This study aims to investigate Tamarindus indica. L. chloroplast rbcL gene for ribulose-1, 5-bisphosphate carboxylase / oxygenase, large subunit as the molecular marker to identified the cultivar level of Tamarindus indica. L. in Thailand. The partial rbcL gene of 19 cultivars were sequenced approximately 611 - 712 bp. The sequence alignment and Phylogenetic Tree analysis were generated byClustalWandNJmethods.The result of nucleotide sequence alignment of partial rbcL gene for all cultivars were divided into 19 groups. The different of theses group similar to the different of local name of Tamarind cultivars in Thailand. Base on these results indicated that 19 cultivars DNABarcodes can supported the local opinions act as the scientific data to identification of tamarind cultivars in Thailand. Keywords : DNA Barcodes, Tamarindus indica. L., RbcL gene, Ribulose-1, 5-bisphosphate carboxylase / oxygenase, Rubisco 1. Introduction Tamarinds are the symbol and economic agricultural plant products of Phetchabun Province, Thailand due to their usefulness. There are so many cultivars of Tamarinds inThailand with the same scientific name: Tamarindus indica. L. such as: Prakaitong, Srichompoopling, Srichompooklom, Preao–puenmuang, Sangartit, Sripakdee, Muenjong, Phetbourtong, Preao – yak, Khunteesoy, Khuntee yourk, Praroj, Numpueng etc. These are named by the farmers and the merchants so that the same cultivar had two or three names. Confusion happened in the using purpose for food or medicinal. Another confusion was planting purpose as too many young tamarind plants were faked. Furthermore, the world climate is changing, the tamarinds in Thailand are contaminated with fungi. The complete automated procedure for systematic, crop improvement or gene variation studying is necessary. Molecular data base is performed as DNA Barcodes.This research project aims to performPhylogenetic Tree reconstruction. Simultaneously, a single nucleotide is analyzed for using asmolecularmarker to diagnosis, classification and cultivar identification for tamarinds in Thailand. 2. Materials and Methods 2.1 Samples Around 1,900 tamarind seeds of different cultivars were used in this study. The leaves of 7 days of seedling were collected and kept at –20 for DNA analysis.