59-05-032 Proceeding

201 Proceedings of the Princess Maha Chakri Sirindhorn Congress cultures was observed by strong Alizarin Red staining in the induction medium whilst absent in control culture. Lipid droplets were detected in adipogenetic culture by red staining revealed byOil Red O whilst not seen in the control culture. Extracellular matrix was observed in chondrogenic sections and stained blue with Toluidine blue, whereas no extracellular matrix was observed in the control pellets (Figure 3). Markers associated with osteogenic, adipogenic, and chondrogenic differentiation were expressed in the induction cultures and not control cultures as detected by reverse transcription-polymerase chain reaction. IV. Discussion This study reveals that cells capable of tri-lineage differentiation are found within the ligamentum flavum, interspinous ligaments, and facet joints. These cells fulfill the criteria for MSCs, capable of forming colonies, showing high-proliferation, tri-lineage differentiation, and they display themarkers frequently associatedwithMSCs [4]. Found in fairly lownumbers compared to conventional MSCs sources they might not be suitable for therapeutic applications and they most likely serve asmaintenance and repair cellswithin the spinal joints. Notwithstanding, their functions in pathological conditions has not yet been fully elucidated and they merit further investigation. V. Significance The present study demonstrates that MSCs can be found in various spinal tissues. They might play an imperative role in the progression of lumbar spinal stenosis. Acknowledgement The authors would like to thankNapaphat Jirathanathornnukul,WanvisaUdomsinprasert and Natthaphon Saetan. References Pittenger MF, et al. Science. 1999; 284:143 - 7. Karp JM, et al. Cell Stem Cell. 2009; 4(3): 206 - 16. Harada Y, et al. Biochemical and Biophysical Research Communications. 2014; 443(3): 1014 - 20. Dominici M, et al. Cytotherapy. 2006; 8(4): 315 - 7.