59-05-032 Proceeding

100 Proceedings of the Princess Maha Chakri Sirindhorn Congress Table 1 FREQUENCY AND SPECTRUMOF VIABLE MUTATIONS INM2 GENERATION Treatments M2 population Spectrum of mutation Total frequency of mutation (%) Leaf type Plant type Seed type Early Brix % 100 Gy 210 1.44 - - - - 1.44 200 Gy 208 0.48 0.48 0.96 - - 1.92 300 Gy 230 1.43 - 0.86 0.43 1.29 4.01 400 Gy 215 1.84 1.38 1.38 1.38 0.46 6.44 500 Gy 210 1.44 0.48 0.48 0.48 - 2.88 600 Gy 214 0.47 0.47 0.47 0.47 0.94 2.82 700 Gy 211 0.94 0.47 - - - 1.41 800 Gy 206 - 0.96 - - - 0.96 Total 1704 Mean 1.0 0.53 0.52 0.34 0.34 2.73 Table 2 QUANTITATIVE TRAITS OF MUTANTS SELECTED IN M2 GENERATION Control/ Mutant Doses Plant height (cm) Stem width (cm) Stalk weight (g) Juice volume (ml) Brix (%) No. of seeds per plant 100 seed weight (g) Yield per plant (g) Days to maturity Control 0 Gy 215.3 10.5 404.0 70 12.6 863 2.06 17.78 126 Narrow Leaf 400 Gy 223.2 10.0 412.2 51 12.5 1064 1.79 19.04 128 Early 400 Gy 221.2 9.20 396.8 60 17.5 858 1.98 16.99 116 Small seed 600 Gy 218.6 11.0 412.1 60 17.5 1648 1.08 17.80 130 Big seed 300 Gy 239.0 12.3 758.6 116 18.6 938 2.82 26.45 121 White spotted 400 Gy 191.16 10.5 395.1 59 14.6 307 2.77 8.50 120 Brown seed 500 Gy 195.15 10.6 407.3 61 14.5 721 1.83 13.20 123 Dwarf 500 Gy 125.2 11.5 363.2 53 13.8 778 1.84 14.31 127 Compact leaf 300 Gy 182.5 13.0 380.0 55 14.5 1083 1.95 21.12 128 High Brix 300 Gy 248.9 12.0 812.4 142 23.4 993 2.60 25.82 121 CV% 16.61 10.35 34.85 42.28 21.01 36.48 25.56 30.33 3.61 SE 10.89 0.362 52.26 9.72 1.06 106.7 0.17 1.74 1.41 CD (p = 0.05) 24.63 0.829 118.2 21.99 2.40 241.4 0.38 3.93 3.20 CD (p = 0.01) 35.38 1.178 169.8 31.59 3.44 346.9 0.54 5.64 4.60 IV. Conclusions The selected mutants that had some promising traits for bioethanol production had been developed. The highest frequency of leaf type, plant type, seed type and early mutant were found in 400 Gy. The high brix mutant at 300 Gy showed the highest variation in stalk weight, juice extraction and brix (%) value with nearly 2 fold better than control. The early mutant matured earlier (10 days) inmaturity compared to the parent cultivar. The early maturedmutants can be of